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Matsunami Glass collagen coated micro cover glass
Collagen Coated Micro Cover Glass, supplied by Matsunami Glass, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagen+coated+micro+cover+glass/coated+collagen/pm42032884-77-4-8
Average 86 stars, based on 1 article reviews
collagen coated micro cover glass - by Bioz Stars, 2026-11
86/100 stars

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Cell Culture:

Article Title: The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes.
Article Snippet: .. HeLa cells cultured on collagen-coated micro cover glass (Matsunami, Osaka, Japan) were fixed with 4% paraformaldehyde (PFA) for 10 min, permeabilized with 0.1% Triton X-100 for 3 min, and blocked with 3% calf serum solution in PBS for 30 min. For the immunofluorescence assay of endogenous RNF115 and GFP-Gal3, cells were first fixed with 4% PFA for 15 min, followed by methanol fixation at 4°C for 5 min, and then blocked with 3% calf serum in PBS for 30 min. After that, cells were incubated with primary antibodies at 4 °C overnight. .. Subsequently, the samples were incubated with secondary antibody Alexa FluorR488 goat anti-mouse/rabbit IgG and Alexa FluorR594 goat anti-mouse/rabbit IgG for 1 h. Cover glasses were mounted on glass slides using Fluoromount mounting medium (Diagnostic BioSystems, Pleasanton, CA, USA).

Immunofluorescence:

Article Title: The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes.
Article Snippet: .. HeLa cells cultured on collagen-coated micro cover glass (Matsunami, Osaka, Japan) were fixed with 4% paraformaldehyde (PFA) for 10 min, permeabilized with 0.1% Triton X-100 for 3 min, and blocked with 3% calf serum solution in PBS for 30 min. For the immunofluorescence assay of endogenous RNF115 and GFP-Gal3, cells were first fixed with 4% PFA for 15 min, followed by methanol fixation at 4°C for 5 min, and then blocked with 3% calf serum in PBS for 30 min. After that, cells were incubated with primary antibodies at 4 °C overnight. .. Subsequently, the samples were incubated with secondary antibody Alexa FluorR488 goat anti-mouse/rabbit IgG and Alexa FluorR594 goat anti-mouse/rabbit IgG for 1 h. Cover glasses were mounted on glass slides using Fluoromount mounting medium (Diagnostic BioSystems, Pleasanton, CA, USA).

Incubation:

Article Title: The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes.
Article Snippet: .. HeLa cells cultured on collagen-coated micro cover glass (Matsunami, Osaka, Japan) were fixed with 4% paraformaldehyde (PFA) for 10 min, permeabilized with 0.1% Triton X-100 for 3 min, and blocked with 3% calf serum solution in PBS for 30 min. For the immunofluorescence assay of endogenous RNF115 and GFP-Gal3, cells were first fixed with 4% PFA for 15 min, followed by methanol fixation at 4°C for 5 min, and then blocked with 3% calf serum in PBS for 30 min. After that, cells were incubated with primary antibodies at 4 °C overnight. .. Subsequently, the samples were incubated with secondary antibody Alexa FluorR488 goat anti-mouse/rabbit IgG and Alexa FluorR594 goat anti-mouse/rabbit IgG for 1 h. Cover glasses were mounted on glass slides using Fluoromount mounting medium (Diagnostic BioSystems, Pleasanton, CA, USA).



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Matsunami Glass collagen coated micro cover glass
Collagen Coated Micro Cover Glass, supplied by Matsunami Glass, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagen+coated+micro+cover+glass/coated+collagen/pm42032884-77-4-8
Average 86 stars, based on 1 article reviews
collagen coated micro cover glass - by Bioz Stars, 2026-11
86/100 stars
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